Table 1.
Compositions of glycerosomes and the results of the U*7(74) uniform design experiments (n=3)
| Experiment number | Factors
|
Responses
|
|||||
|---|---|---|---|---|---|---|---|
| A (w/v, %) | B (w/v, %) | C (v/v, %) | Mean size (nm) | PDI | Zeta potential (mV) | EE (%) | |
| 1 | 5.0 | 0.05 | 30 | 344.32±11.19 | 0.17±0.04 | −7.01±0.13 | 57.56±1.57 |
| 2 | 4.5 | 0.1 | 15 | 182.70±4.85 | 0.36±0.01 | −13.6±0.36 | 50.46±0.31 |
| 3 | 4.0 | 0.2 | 40 | 272.23±2.62 | 0.28±0.02 | −9.04±0.69 | 44.03±2.00 |
| 4 | 3.5 | 0.3 | 25 | 267.97±2.16 | 0.27±0.01 | −9.84±0.27 | 46.23±6.75 |
| 5 | 3.0 | 0.4 | 10 | 179.33±11.06 | 0.37±0.03 | −17.93±0.51 | 41.11±0.71 |
| 6 | 2.5 | 0.5 | 35 | 368.75±16.70 | 0.41±0.02 | −6.70±0.12 | 43.44±1.84 |
| 7 | 2.0 | 0.6 | 20 | 245.25±5.18 | 0.35±0.03 | −15.47±0.51 | 31.11±0.47 |
Notes: Factor A was the concentration of phospholipid, factor B was the concentration of cholesterol, and factor C was the amount of glycerol in water. The experimental responses included particle size, polydispersity index (PDI), zeta potential, and encapsulation efficiency (EE).