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. 2015 Oct 16;6:8651. doi: 10.1038/ncomms9651

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Unmethylated or chemically methylated p53 peptides were incubated with the endogenous SETDB1 complex. The end products were analysed using LC-MS/MS to confirm the p53 methylation. Corresponding peptide sequences and methylation status were marked by the identified peaks. (a) p53K370me0 peptide was used as the substrate. (b) K370me1 peptide was used as the substrate. (c) SMYD2 was used to induce K370me1. SETDB1 mainly catalyses p53K370me1 to K370me2. (d) SMYD2 alone with the p53K370me0 peptide was used as a control.