Figure 2.
Densin does not cause CDF but increases current density of Cav1.2 in HEK293T cells. A, ICa was evoked by 5 ms pulses from −80 to 10 mV at 100 Hz in cells transfected with Cav1.2 alone (n = 13) or cotransfected with densin and CaMKII (n = 11). Test current amplitudes were normalized to the first in the train (Fractional I) and plotted against time (right). B, ICa was evoked by 50 ms depolarizations from −80 mV to various voltages and normalized to cell capacitance. Representative current traces and plots of current density against voltage are shown for cells transfected with Cav1.2 and: eGFP (n = 13), GFP-densin (n = 15), and GFP-densin + CaMKII (n = 11). C, Same as in B except for ICa recorded in cells transfected with Cav1.2ΔB1a and eGFP (n = 14) or GFP-densin (n = 14).