Figure 4.
Densin does not affect internalization of Cav1.2 in transfected HEK293T cells. A, Cells expressing Cav1.2-HA and eGFP (top) or GFP-densin (bottom) were incubated with HA antibodies to label cell surface channels and incubated for the indicated times (in minutes) to allow for channel internalization. The cells were then fixed and processed with Cav1.2 antibodies to label total channel protein. Scale bars, 10 μm. B, Cell surface HA fluorescence (arbitrary units, a.u.) was plotted against internalization time. Results are representative of three independent experiments. C, Same as B but HA intensity was normalized to that at time = 0 min. Smooth line represents fit with a single exponential equation.