Figure 4.

Regulation of Gad1 promoter activity by small molecules that stabilize nucleic acid secondary structure. A, PCR interference assays showed that increasing concentrations of TMPyP4, but not TMPyP2, blocked transcription and PCR amplification of WT template oligonucleotides. *p < 0.01. B–G, Gad1-GFP expression was strongly repressed in the main OB (MOB) of depolarized forebrain slice cultures after 24 h exposure to 100 μm of TMPyP4, but not TMPyP2. Gad1-GFP is not expressed in the accessory OB (AOB), and Gad1-GFP expression in the rostral migratory stream (RMS) does not show the same sensitivity to TMPyP4. Scale bar, 100 μm.