Figure 9.

Differential occupancy of G-quadruplexes and hnRNPs on the Th and Gad1 promoters in response to membrane depolarization of cultured cells. A, B, Relative Th and Gad1 expression levels, respectively, as measured by qRT-PCR, were increased in SH-SY5Y (A) and NT2 (B) cells depolarized with 50 mm KCl compared with control cultures treated with 50 mm NaCl. C, D, ChIP assays with the BG4 antibody detected significantly more G-quadruplex secondary structure on the Th and Gad1 promoters in the SH-SY5Y and NT2 cells, respectively, depolarized with KCl compared with control cultures treated with NaCl. E, F, ChIP assays showed a significant increase in hnRNP K occupancy on the Th promoter in depolarized SH-SY5Y cells compared with NaCl-treated control cells. F, ChIP assays showed a significant increase in hnRNP LL occupancy on the Gad1 promoter in depolarized NT2 cells compared with NaCl-treated control cells. *p < 0.01.