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. 2016 May 13;30(7):693–708. doi: 10.1210/me.2015-1299

Figure 6. PRL induces invasiveness of T47D cells in a NHE1-dependent manner.

Figure 6.

Invasiveness of MCF10A (A) and T47D (A and B) cells was assessed in matrigel-coated Boyden chambers. PRL (10 nM) was applied as a chemoattractant to the lower chamber, as indicated, and where indicated, EIPA (5 μM) (A) or cariporide (10 μM) (B) was applied to both chambers to inhibit NHE1. Data are shown as relative cell invasion (invasion relative to migration) in the presence or absence of PRL and/or EIPA or cariporide as indicated. Error bars represent SEM of four (MCF10; A), five (T47D; A), or three (T47D; B) independent experiments. *, P ≤ .05, **, P ≤ .01, ***, P ≤ .001, one-way ANOVA with Tukey posttest. Ctrl, control.