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. 2016 May 4;30(7):748–762. doi: 10.1210/me.2016-1001

Figure 1. Glucose exerts specific effects on CRE-dependent reporter gene activity in mHypoA-2/10 cells.

Figure 1.

A and B, Reporter activity was measured after transient expression of a CRE-, NFAT-, or STAT-dependent reporter construct. A, Cells were cultured with the indicated glucose concentration for 24 hours. Data of 5 independent experiments performed in quadruplicates are presented as the mean ± SEM. Asterisks indicate a significant difference to the next lower glucose concentration. B, Cells were cultured without glucose for the indicated period of time. Data of 3 independent experiments performed in quadruplicates were compiled by setting the corresponding reporter activity measured at time point 0 to 100% and expressed as the mean ± SEM. Hash signs indicate a significant difference to 100%. C, mHypoA-2/10-CRE cells stably expressing the CRE-dependent reporter were cultured with the indicated glucose or pyruvate concentration for 24 hours. Data of 15 independent experiments performed in quadruplicates are presented as the mean ± SEM. Asterisks indicate a significant difference to the values of 25mM glucose. D, mHypoA-2/10-CRE cells were cultured with 1.0mM glucose or fructose-1,6-bisphophate for 24 hours. Data of 5 independent experiments performed in quadruplicates are presented as the mean ± SEM. Asterisks indicate a significant difference to the values of 25mM glucose.