Fig 1. Characterization of the BP2936 locus by RNA seq.

A. Representation of the deep RNA sequencing results for the locus encompassing BP2936. BP2936 is encoded by the anti-sens strand of the genome. The red and green bars under the genes represent transcripts of the anti-sense and sense strands, respectively. B. Differential RNA sequencing was used to identify the transcription initiation site of BP2936. The consensus sequence is indicated. Translation of the coding DNA sequence is shown from right to left, indicating the two possible initiation Met. The primary transcripts identified by dRNA seq show that the transcription start site maps between these two codons, at nucleotide position 3125512 in the genome of Tohama I.