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. 2017 May 11;12(5):e0176396. doi: 10.1371/journal.pone.0176396

Fig 2. Detection of the product of BP2936 by immunoblot analyses.

Fig 2

(A), insoluble and soluble fractions of BPSM (wt control; lanes 1 and 4), BPRM1 (deletion mutant; lanes 2 and 5) and BPRM1(pBP2936) (mutant complemented on plasmid; lanes 3 and 6) were analyzed by immunoblotting with antibodies raised against the recombinant protein. (B), the same experiment was performed with the insoluble and soluble fractions of BPSM (lanes 1 and 3) and BPRM2 (mutant complemented on chromosome; lanes 2 and 4). (C), a comparison of the sizes of the three proteins, i.e., the 2936P protein produced by B. pertussis (lane 1), and the recombinant proteins rec2936P-sh (lane 2) and rec2936P-lg (lane 3) produced in E. coli, was performed. Note that 2936P migrates slightly faster than the shorter recombinant protein, most likely because of the purification tag added to the latter.