Table 3. Parameters analyzed in the studies demonstrate the effects of high-fat diet and alcohol on the cutaneous repair in murine models.

High Fat Diet
Reference	Wound healing rate	Extracellular matrix components analyzed	Weight	Glucose test
Pence et al., 2012 [35]	Mice fed with High fat diet (HFD) showed impaired wound healing and larger wound sizes. However, wound size was significantly smaller in exercised obese mice, compared with HFD-sedentary and chow-sedentary groups	?	HFD, body weight was significantly higher, compared to the control group.	The HFD group exhibited elevated levels of blood glucose, when compared to chow-exercise, and chow-sedentary groups.
Hiebert et al., 2013 [36]	HFD exhibited reduced wound closure, delayed contraction; and chronic inflammation, compared with the control group.	The HFD-fed mice presented less collagen and decorin, and less closing wounds compared with the controls. Animals treated with the Granzime B and HFD presented increases the fibronectin and vitronectin compared with the Wild-type animals.	ApoE knouchout (AKO) mice fed a HFD presented increased weight when compared to AKO mice fed a different diet.	?
Otranto et al., 2012 [37]	The wound area was 27% greater in the high-fat chow group on day 7 and 110% greater 14 days after wounding, when compared with the standard chow (SC) group.	The collagen fibers were less organized and less dense in the HFC group. The hydroxyproline levels were lower in the high-fat chow (HFC) group.	From the 8th week, the HFC group presented higher body weight.	The blood glucose of the HFC group was higher than the standard chow (SC) group
Nascimento & Costa, 2006 [38]	21 days after wounding, the wound area in the fat diet group was 32% larger, which indicates less contraction in relation control group	Higher collagen density was observed in the control group, compared to the fat diet group.	In the fat diet group, the body weight gain was significantly greater than in the control group.	Blood concentration of glucose was not different between the groups throughout the experiment.
Gupta et al., 2015 [39]	Fentanyl treatment resulted in complete reepithelization and dense granulation tissue in the wound scars on day 36 in diabetic rats treated with high fat diet.	There was increased collagen content in fentanyl treated wounds, compared to PBS in diabetic rats treated with high fat diet.	?	?
Seitz et al., 2015 [40]	Mice fed HFD showed increased wound area and incomplete wound epithelialization in the end of the experiment, compared mice which had received a standard chow diet (CD)	?	The uptake of the HFD caused a significant increase in body weight.	The HFD group exhibited elevated levels of blood glucose.
Slavkovsky et al., 2011 [41]	Animals fed high-fat diet showed significantly increased scar size: by 40% in males, and 140% in females.	The levels of hydroxyproline, tropoelastin and procollagen were reduced in diabetic groups (HFD). MMP3 and MMP13 (matrix metalloproteinase) and MPO (myeloperoxidase) levels increased in animals fed HFD.	The animals fed HFD showed increased body weight in both sexes.The difference was more pronounced in females, twice the value of the control.	The high-fat diet animals group, presented elevated glucose levels, when compared to control.
Holcomb et al., 2009 [42]	HDF fed animals and those ovarectomized (OVX) presented 61% of the wound open, when compared to No ovarectomized (NOVX) and HFD fed mice, which presented 34% of the wound open.	?	OVX mice treated with high-fat diet presented higher final body weight than NOVX mice treated with HFD.	?
Nascimento & Costa 2011 [43]	The diet-induced obesity (DIO) group, showed no sign of reepithelialization. The percentage of the reepithelialization wound area was lower in the DIO groups compared with the control group.	In the diet-induced obesity (DIO) and diet-resistant (DR) groups, hydroxyproline levels were lower 7 days after wounding and increased slightly 14 days after wounding.	The diet-induced obesity (DIO) group presented a higher average body weight, than the diet-resistant (DR) group.	The blood glucose level of the diet-resistant (DR) group was lower than the glucose levels of the control and diet-induced obesity (DIO) groups.
Leite et al., 2015 [44]	The wound healing rate was reduced in animals treated with hyperlipidic diet and laser off.	The hydroxyproline content was reduced in the groups treated with the hyperlipidic diet and laser off.	There was no difference in the body mass of the animals after 45 days on a high-fat diet.	A higher serum glucose level, were observed in hyperlipidic animals.
Alcohol
Reference	Histopathological evaluation	ECM components analyzed	Cytokines	Weight
Choudhrya et al., 2000 [45]	It was observed decreased proliferation of splenocytes derived from animals subjected to the combination of alcohol and burn injury.	?	A 50% decrease in IL-2 production was observed by splenocytes derived from burn animals compared with the splenocytes from sham animals
Faunce et al., 2003 [46]	The neutrophil content of the skin of mice after burn injury was not significantly difference with of ethanol treatment, when compared to not burns (Sham+Vehicle and Sham+Ethanol) groups.	?	Myeloperoxidase (MPO) content increased in the groups Burn+Vehicle and Burn+Ethanol when compared to Sham+Vehicle and Sham+Ethanol. The production of TNF-α was lower in the Burn+Ethanol groups compared with Burn+Vehicle groups	?
Radek et al., 2005 [47]	Reepithelialization was lower in the groups treated with the Ethanol, when compared to the control groups, but does not inhibit keratinocyte migration across the wound bed.	Hydroxyproline, was significantly reduced at day 7, in wounds from ethanol-treated animals compared with the control.	The level of FGF-2 was lower in wounds from ethanol-treated mice and VEGF levels were significantly higher in wounds from ethanol-treated mice compared with the control.	?
Ranzer et al., 2011 [48]	Exposure to ethanol decreased of fibroblast proliferation, and impairment on the regulatory function of fibroblasts when compared to control.	The levels of collagen and hyaluronic acid of the wounds in mice exposed to ethanol were significantly reduced compared to control.	Lysyl Oxidase activity (LOX) in the wounds of the mice treated with ethanol decreased significantly compared to control groups.	?
Fitzgerald et al., 2007 [49]	The histological examination of wounds by myeloperoxidase (MPO) reveals a reduction at 12 and 24hs of the neutrophil infiltration. The analysis of the macrophage inflammatory protein-2 (MIP-2) reveal a reduction at 12hs, post injury in the groups treated with the ethanol	?	TNFα levels were unchanged after injury in both groups ethanol and saline-treated. IL-1β showed variables peak levels, with reduction at 6 and 12 hs and high after 24hs in the groups treated with the ethanol.