Table 1. Shown are primer structure (5’-3’), references and expected PCR fragment size in base pair (bp).
| Primers | PCR Band | References |
|---|---|---|
| PR1 | 300 bp | NM_001113335.1 |
| TTCTCAAAGAAAGCCTCACCA | ||
| [2341–2361 bp; Tm 60°C] | ||
| PR2 | ||
| TTTGGCTTCATATCATGGCTTT | ||
| [2619–2640 bp; Tm 60°C] | ||
| PR3 | 175 bp | |
| TTTGGCTTCATATCATGGCTTTGGAGAGCAGGGGC | ||
| [2481–2493 bp; Tm 46°C] | ||
| PR4 (Hnup) | 325 bp | NM_012653 |
| TGCATATAGCAGTTAAAGTAAG | ||
| [47172974–47172995 bp; Tm 58°C] | ||
| PR5 (Hndown) | ||
| TGGTGAGGCTTTCTTTGAGAA | ||
| [47173278–47173298 bp; Tm 58°C] |
PR1 = Upstream primer, PR2 = Downstream primer used for mRNA amplification, and PR3 = Bridge primer used to prepare a competitive control. The underlined sequence in PR3 is PR2 sequence. Hnup/Down = Heteronuclear RNA upstream (PR4) /downstream (PR5) primers.