Skip to main content
. 2017 May 11;12(5):e0176974. doi: 10.1371/journal.pone.0176974

Fig 5. Inactivated-H5N1 increases phosphorylation of ERK1/2 and p38 MAPK.

Fig 5

Immunoblot analysis of ERK1/2 (A) and p38 MAPK (B) protein expression in 16HBE14o- cells treated with inactivated-H5N1. Cells were untreated or treated with PD98059 (50 μM) or SB203580 (10 μM) as appropriate. One hour later, cells were treated for 30 min with vehicle, allantoic fluid or inactivated-H5N1 before being harvested for immunoblot analysis for the presence of ERK1/2, phospho-ERK1/2 (P-ERK1/2), p38 MAPK or phospho-p38 MAPK (P-p38). Upper panel, representative gels for immunoblot analysis of ERK1/2 and phosphorylated ERK1/2 (P-ERK1/2) (A) or p38 MAPK and phosphorylated p38 MAPK (P-p38) (B). Lower panel, quantitative analysis of the amount of P-ERK1/2 (A) or P-p38 MAPK (B), expressed as fold change, using ImageJ software (NIH). Values are means ± SEM from at least 3 sets of experiments. * and *** indicates p < 0.05 and p < 0.001, respectively (one-way ANOVA with Student-Newman-Keuls post-hoc test).