PPP1R3G overexpression improves hepatic steatosis under fasting conditions. (A) The expression of PPP1R3G protein in the liver of 4-month-old male wild-type and transgenic mouse in overnight-fasted state. These animals were used in measurement of hepatic glycogen content (B, n = 8 for each group), liver GS activity (C, n = 6 for each group), PAS staining (D), Oil-Red-O staining (E), measurement of hepatic triglyceride level (F, n = 7 for wild-type and n = 8 for transgenic mice). (F) Serum parameters in wild-type and the transgenic mice after overnight fasting (n = 8 for per group).The data are shown as mean ± SEM. *, P < .05; **, P < .01.