Figure 6.

PPP1R3G overexpression increases glycogen level in primary hepatocytes. Primary hepatocytes isolated from wild-type and the transgenic mice were cultured overnight and used in the following experiments: analysis of PPP1R3G protein expression by immunoblotting (A), measurement of cellular glycogen content (B), cellular glycogen concentration after treatment for 24 hours with various glucose concentrations (C), and measurement of glycogen content after treatment with 100 nM insulin for 24 hours (D). The data are shown as mean ± SD (Student's t test for B and ANOVA for C and D). **, P < .01 as compared with wild-type and the transgenic mice with the same treatment. ^^, P < .01 as compared with the first group of mice with the same genotype.