Figure 7.

The glycogen binding domain is required for the physiological activities of PPP1R3G. (A) Analysis of PPP1R3G protein in the liver of 9-month-old male wild-type and the transgenic mice that expressed PPP1R3G containing deletion of glycogen binding domain (ΔGB) in the fed state. (B) Measurement of hepatic glycogen content (n = 5 for wild-type and n = 8 for the transgenic mice, respectively, the same for the following experiments). Blood glucose level was analyzed in the fed state (C) and in the overnight-fasted state (D). Glucose tolerance test (E) and food tolerance test (F) were performed in wild-type and the ΔGB transgenic mice. Hepatic triglyceride was measured in the fed state (G). Data are shown as mean ± SEM.