Effects of IGF-I on the expression of kitlga in cultured zebrafish follicle cells or intact follicles. A, Time course of IGF-I effect (50 ng/mL) on the expression of kitlga in cultured zebrafish follicle cells. B, Dose response of IGF-I effect on kitlga expression in cultured zebrafish follicle cells after 6 hours of treatment. C, Dose response of IGF-I effect on the expression of kitlga in intact EV follicles after 6 hours of treatment. The data were expressed as the percentage or fold change of the respective control group after normalization to the expression of the housekeeping gene, ef1a (mean ± SEM; n = 4). *, P < .05; ***, P < .001 vs control.