Fig 6. Action of tyrosinase on α-arbutin in the presence of MBTH.

The experimental conditions were [E]₀ = 300 nM, [MBTH]₀ = 0.2 mM, [α-arbutin]₀ = 10 μM and DMF 2%. The spectrophotometric recordings were made every 60 seconds. Inset. Determination of the MBTH saturation concentration. The experimental conditions were [E]₀ = 100 nM, [α-arbutin]₀ = 20 mM and DMF 2%.