Table 3. Common SNO protein identifications resulting from different cardioprotective interventions.
Protein Name | Protein ID | SNO Cysteine | |||||
---|---|---|---|---|---|---|---|
Male Baseline |
Female Baseline |
Male CHA |
Female CHA |
Male [3] IPC |
Male [4] PostC |
||
Aconitase | Q99KI0 | 385 | 385 | 385 | 385 | 385 | 385 |
Aspartate aminotransferase, mitochondrial | P05202 | 295 | 295 | ND | 295 | 295 | 295 |
Cytochrome b-c1 complex subunit 1, mitochondrial | Q9CZ13 | 268, 380 | 268, 380 | 268, 380 | 268, 380 | 268, 380 | 380 |
Electron transfer flavoprotein subunit alpha, mitochondrial | Q99LC5 | 53, 155 | 53, 155 | ND | 155 | 155 | ND |
Electron transfer flavoprotein subunit beta, mitochondrial | Q9DCW4 | 71 | 71 | 71 | 71 | 66, 71 | ND |
Enoyl-CoA hydratase, mitochondrial | Q8BH95 | 225 | 111, 225 | 111, 225 | 111, 225 | 225 | 111, 225 |
Fructose-bisphosphate aldolase A | P05064 | 339 | 339 | 339 | 339 | 339 | 339 |
Glyceraldehyde-3-phosphate dehydrogenase | P16858 | 150, 154, 245 | 150, 154, 245 | 150, 154, 245 | 150, 154, 245 | 150, 154, 245 | 150, 154, 245 |
Isocitrate dehydrogenase [NAD] subunit alpha | Q9D6R2 | ND | ND | 359 | 359 | 331 | 331 |
Isocitrate dehydrogenase [NADP], mitochondrial | P54071 | 113, 402 | 113, 402 | ND | 113, 402 | 308, 402 | 308, 402 |
L-lactate dehydrogenase A chain | P06151 | 84, 163 | 84, 163 | 84, 163 | 84, 163 | 84, 163 | 163 |
Malate dehydrogenase, cytoplasmic | P14152 | 137, 154 | 137, 154 | 137 | 137, 154 | 137 | ND |
Malate dehydrogenase, mitochondrial | P08249 | 89, 93, 275, 285 | 89, 93, 211, 275, 285 | 89, 93, 275 | 89, 93, 211, 275, 285 | 275 | 89, 275 |
Mitochondrial complex I-75 kDa | Q91VD9 | 75, 92 | 75, 92 | 75, 92 | 92, 726 | 92 | 75 |
Myosin light chain 1 | P09542 | 191 | 85, 191 | ND | 85, 191 | ND | ND |
Propionyl CoA carboxylase alpha chain | Q91ZA3 | 107 | 107 | 107 | 107 | 107 | 107 |
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 | O55143 | 349, 364, 447, 471 | 344, 349, 364, 447, 471, 635, 669 | 364, 635 | 344, 364, 447, 635, 998 | 498 | 344, 349, 364 |
Serum albumin | P07724 | 77, 289, 416, 500, 501 | 77, 289 | 77, 289 | 77, 289 | ND | ND |
Succinate dehydrogenase [ubiquinone] flavoprotein, mitochondrial | Q8K2B3 | 536 | 89, 536, 654 | 654 | 536, 654 | 536 | 654 |
Succinyl-CoA ligase alpha | Q9WUM5 | 172, 181 | 172, 181 | 172, 181 | 172 | 60 | 172, 181 |
Succinyl-CoA ligase subunit beta, mitochondrial | Q9Z2I9 | 430 | 152, 158, 270, 430 | 152, 158, 430 | 152, 158, 430 | 430 | 158, 430 |
Triosephosphate isomerase | P17751 | 117, 268 | 117, 268 | 117, 268 | 117, 268 | 67, 218 | 117, 268 |
Voltage-dependent anion-selective channel protein 1 | Q60932 | 245 | 245 | 245 | 245 | 245 | 245 |
Voltage-dependent anion-selective channel protein 2 | Q60930 | 48 | 48 | 48 | 48 | 48 | 48 |
Common SNO protein identifications resulting from different cardioprotective interventions (i.e., ischemic pre- and post-conditioning). SNO proteins were identified in male and female hearts (as noted on the top of the table), using SNO-RAC in tandem with LC-MS/MS. ‘SNO cysteine’ represents the modified residue; ‘ND’ indicates that the protein was not identified in the given sample set.