Fig 3. SPL decreases diabetes-induced oxidative stress in GL and PT.
To evaluate oxidative stress, superoxide anion (O2●―) production, lipid peroxidation, protein carbonylation and reduced glutathione (GSH) content were measured in isolated GL and PT. SPL treatment prevents diabetes-induced increment in O2●― production (A) by using nicotinamide adenine dinucleotide phosphate (NADPH) as substrate and, diphenyleneiodonium (DPI) as inhibitor, lipid peroxidation (B) and protein carbonylation (C) and decreased GSH content (D) in GL (S3 Fig). Also, SPL diminished diabetes-induced increment of O2●― production (E), lipid peroxidation (F) and protein carbonylation (G) and decreased GSH content (H) in PT (S3 Fig). Similar results were found between CTL and SPL groups. Data are mean±SEM from 5–6 rats per group. *p<0.05; **p<0.01 and ***p<0.001.