Fig 5. SPL treatment decreases diabetes-induced co-immunoprecipitation (co-IP) of cldn-4 and -8.

To evaluate direct interaction of cldn-4 and -8, co-IP analyzes from isolated DT from the four experimental groups were performed. As shown in panel A, cldn-4 co-immunoprecipitated with cldn-8; densitometric analysis (B) shows that diabetic condition increased their interaction and SPL treatment prevents this change (S5 Fig). Also, increased co-IP of cldn-8 with cldn-4 was found under diabetic condition and SPL decreased this interaction (C), densitometric analysis show that these changes were significant (D) (S5 Fig). As shown in panels A and C, no signal was found under nonspecific conditions of IP performed with an unrelated antibody. GAPDH was used in input extract as loading control. Data are mean±SEM from 3 rats per group. *p<0.05.