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. 2017 May 11;12(5):e0177362. doi: 10.1371/journal.pone.0177362

Fig 7. SPL treatment decreases diabetes-induced increased WNK4 expression and co-IP with cldn- 4 and -8.

Fig 7

WNK4 expression was evaluated by IB and qRT-PCR in the four experimental groups (S6 Fig). As shown in panels A and C, diabetes induced WNK4 protein expression and mRNA levels, respectively. SPL treatment decreased these changes. Densitometric analysis of IB is shown in panel B. To evaluate direct interaction of WNK4 with cldn-4 and -8 at the TJ, co-IP analyzes were performed. As shown, diabetes induced co-IP of WNK4 with cldn-4 (D) and vice versa (E). Also, diabetic condition induced co-IP of WNK4 with cldn-8 (F) and vice versa (G). SPL treatment decreased these changes. As shown in panels D-G, no signal was found under nonspecific conditions of IP performed with an unrelated antibody. GAPDH was evaluated in isolated DT and input extract as loading control. Data are mean±SEM from 3 rats per group. *p<0.05 and ***p<0.001.