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. 2017 May 11;12(5):e0177362. doi: 10.1371/journal.pone.0177362

Fig 8. Diabetic condition induces phosphorylation of cldn-4 and -8 in threonine but not in serine residues.

Fig 8

Phosphorylation of cldn-4 and -8 in threonine and serine residues was evaluated by IP assays in the four experimental groups. As shown, diabetes induced cldn-4 (A) and cldn-8 (B) phosphorylation in threonine, but not in serine residues. SPL treatment decreased these changes. As shown in panels A and B, no signal was found under nonspecific conditions of IP performed with an unrelated antibody. GAPDH was evaluated in input extract as loading control.