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. 2017 May 11;12(5):e0177362. doi: 10.1371/journal.pone.0177362

Fig 9. SPL treatment ameliorated diabetes-induced increased expression of SGK1, WNK4 and co-IP of WNK4 with SGK1.

Fig 9

SGK1 expression was evaluated by IB analysis of isolated DT. As shown in panel A, DBT induced SGK1 expression. Densitometric analysis is shown in panel B (S7 Fig). Diabetic condition also induced co-IP of WNK4 with SGK1, and serine, but not threonine phosphorylation of WNK4 (C). SPL treatment prevented these changes. GAPDH was used as loading control in isolated DT and input extracts. As shown in panel C, no signal was found under nonspecific conditions of IP performed with an unrelated antibody. Data are mean±SEM from 3 rats per group. **p<0.01.