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. 2017 Feb 13;69(5):286–297. doi: 10.1097/FJC.0000000000000472

FIGURE 3.

FIGURE 3.

Effects of pretreatment of animals with α-MSH and/or the HO-1 inhibitor SnPP on cardiac functions in isolated working hearts (protocol II). Working hearts were isolated from 4 groups of rats which had been pretreated with injections of α-MSH and/or saline—and selected animals receiving injections of the HO-1 inhibitor SnPP, 24 hours before sacrifice and heart isolation. Treatments were as follows: 0.5 mL saline subcutaneously (group II-a, control); 250 μg/kg body weight α-MSH subcutaneously (group II-b, MSH); 50 μg/kg body weight SnPP intraperitonealy, plus 250 μg/kg body weight α-MSH subcutaneously (group II-c, SnPP + MSH); or 50 μg/kg body weight SnPP, intraperitonealy (group II-d, SnPP). Cardiac function parameters were recorded before ischemia (baseline), and at 30, 60, 90, and 120 minutes of reperfusion. Results are shown as average values from each group of rat hearts ± SEM of HR [bpm, (A)]; CF [mL/min, (B)]; AF [mL/min, (C)]; CO[ mL/min, (D)]; and SV [mL, (E)]. *P < 0.05, significant differences between groups. **P < 0.01, significant differences between groups. ***P < 0.001, significant differences between groups.