Table 2. Primers designed for qRT-PCR analysis of flavonoid biosynthesis genes in Brassica campestris ssp. chinensis L.

Gene	Forward primer (from 5’ to 3’)	Reverse primer (from 5’ to 3’)	Tm(°C)
CHS	CATCTGACACCCACCTTGAC	GAAGATGGGCTTCTCTCCAG	58.5
	GAGAGAAGCCCATCTTCGAG	GTCCCACTTCCCTCAAGTGT	57.2
CHI	TCCCTTTCTTCCGTGAAATC	TTCCATATCGCCACACAGTT	54.7
	AACTGTGTGGCGATATGGAA	GAGAGCGAAGAGGATGGAAG	55.7
F3H	GTCCCAAGGTTGCCTACAAT	CCAGTTCTCACAAGCCTCAA	56.1
	ATAGCCACGTTCCAGAATCC	CTCCAAGATCGGCTTCTCTC	57.0
ANS	GGGATCAGCTCTATCCCAAA	GGACTTGTGGACCGTCTTCT	56.5
	GGTTTGCAGCTGTTCTACGA	CACCAATCCACGGTGAAGTA	55.2
FLS	AATTACTATCCGCCGTGTCC	TTGACGTCGATCCAGTGATT	55.5
	ACTTCCGGAATCATCGTCAT	AAACCGGCCATGATATTCTC	56.2

Note: The primers which were finally selected for qRT-PCR analysis were shown in bold font.