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. 2017 May 11;12(5):e0177185. doi: 10.1371/journal.pone.0177185

Table 2. Primers designed for qRT-PCR analysis of flavonoid biosynthesis genes in Brassica campestris ssp. chinensis L.

Gene Forward primer (from 5’ to 3’) Reverse primer (from 5’ to 3’) Tm(°C)
CHS CATCTGACACCCACCTTGAC GAAGATGGGCTTCTCTCCAG 58.5
GAGAGAAGCCCATCTTCGAG GTCCCACTTCCCTCAAGTGT 57.2
CHI TCCCTTTCTTCCGTGAAATC TTCCATATCGCCACACAGTT 54.7
AACTGTGTGGCGATATGGAA GAGAGCGAAGAGGATGGAAG 55.7
F3H GTCCCAAGGTTGCCTACAAT CCAGTTCTCACAAGCCTCAA 56.1
ATAGCCACGTTCCAGAATCC CTCCAAGATCGGCTTCTCTC 57.0
ANS GGGATCAGCTCTATCCCAAA GGACTTGTGGACCGTCTTCT 56.5
GGTTTGCAGCTGTTCTACGA CACCAATCCACGGTGAAGTA 55.2
FLS AATTACTATCCGCCGTGTCC TTGACGTCGATCCAGTGATT 55.5
ACTTCCGGAATCATCGTCAT AAACCGGCCATGATATTCTC 56.2

Note: The primers which were finally selected for qRT-PCR analysis were shown in bold font.