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Raw cells were treated for 24h with: 5 mM D-glucose (LG), or 25 mM D-glucose (HG). (A) ROS levels were measured using the Cellular Reactive Oxygen Species Detection Assay Kit. The data is represented as Mean ± SD, n = 5 per treatment group. (B) RT-qPCR analysis of NOX2 gene expression. Results show increased NOX2 gene expression under high glucose conditions.
