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. 2017 May 11;12(5):e0177453. doi: 10.1371/journal.pone.0177453

Fig 3. Overexpression of Lethe in RAW macrophage cells decreases ROS production.

Fig 3

(A) Overexpression of Lethe gene expression was achieved by plasmid transfection and confirmed by RNA analyses by RT-qPCR (mean ± SD, n = 3 per group), Lethe gene expression was significantly induced in the RAW macrophages transfected with pcDNA-Lethe compared to the RAW macrophages transfected with pcDNA3.1. (B) Under HG condition, ROS production was prevented following transfection of RAW macrophages with pcDNA-Lethe (mean ± SD, n = 5 per group). ROS levels were measured by the Cellular Reactive Oxygen Species Detection Kit.