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. 2017 May 11;12(5):e0177453. doi: 10.1371/journal.pone.0177453

Fig 6. Lethe overexpression prevents translocation of p65 to nucleus.

Fig 6

(A) Western blot analysis showed that hyperglycemia induced nuclear p65, while overexpression of Lethe significantly reduced nuclear p65. For protein in nuclear fraction, we used TATA binding protein (TBP) as loading control; for protein in cytoplasmic fraction, we used GADPH as loading control. (B) Protein levels were normalized to cytoplasmic GAPDH and nuclear TBP by the software ImageJ. Results indicated that there was no change in total p65 protein level, while Lethe overexpression significantly reduced nuclear p65 (mean ± SD, n = 3 per group), while no significantly difference in total p65 protein levels between groups.