Fig 4. ^99mTc0₄^- uptake in Hela cells transfected with the pRINES/122T plasmid and miRNA mimics.

A) Hela cells were transfected with pRINES/122T in presence of indicated concentrations of miRNA-122 mimic or, as a control, miRNA-133 and -1 mimics. For a set of experiments, sodium perchlorate (NaClO₄), an inhibitor of hNIS-mediated ^99mTc0₄^- uptake, was added to the cells to assess specificity of the results. (B) ^99mTc0₄^- uptake in Hela cells correlated with the number of Hela cells transfected with the pRINES/122T and treated with Cumate (100 μg/ml). Inset is the correlation coefficient value. Data shown are the mean ±SD of one representative experiment performed in triplicate and reproduced at least three times. Statistical analysis in Fig 4A was performed by a two-tailed t-test, * P ≤ 0.05 compared to control cells, not transfected with miRNAs.