Table 2. Phenotypic description of PNs in wild-type, Sema-1a LOF and rescue experiments in Figs 2 and 4 and S7 Fig.
Sema-1a-deficient PNs with a phenotype of dendritic mis-targeting to the region close to the VC1 glomerulus |
type of PN (emb/larval)a |
conditions of Sema-1a expression |
total number (n) | % of dendritic mis-targeting in close proximity of the VC1 glomerulus | % of dendritic occupancy in Brp negative regions and/or partial glomerulus | % of other dendritic mis-targeting | notes |
DC1 adPNe (emb; Fig 2) |
wt | 5 | 0% | 0% | 20%d | d some dendritic arbors around the D glomerulus | |
Sema-1aP1 | 3 | 100%b | 0% | 67%d |
b dendritic innervation to the region dorsal to the VC1 glomerulus d dendritic innervation in the ventrolateral part of the DA3 glomerulus |
||
DP1m adPN (emb; Fig 4) |
wt | 24 | 0% | 0% | 17%d |
d a patch of dendritic arbors in the DM3 (9%) and DM1 (4%) glomeruli; heavy dendritic innervation in both DP1m and VL2p glomerulus |
|
Sema-1aP1 | 34 | 71%b | 0% | 62%d |
b dendritic innervation to the region around the VC1 glomerulus d dendritic innervation in the DM3 glomerulus (56%) and the region lateral to the VM7 glomerulus (26%) |
||
VL2p adPN (emb; Fig 4) |
wt | 43 | 5%b | 0% | 9%d |
b a patch of dendritic arbors ventrally around the VC1 glomerulus d some dendritic arbors posterior to DP1m, V or VL1 glomerulus or posterior to the AL |
|
Sema-1aP1 | 15 | 87%b | 0% | 87%d |
b dendritic innervation mostly to the region posterior to the VC1 glomerulus (13% out of 87% without the SEZ innervation) d dendritic innervation to the SEZ (13% out of 87% without the innervation around the VC1 glomerulus) |
||
DC2 adPN (larval; Fig 4 & S7) |
wt | 5 | 0% | 0% | 40%d | d a patch of dendritic arbors around the VA6 glomerulus | |
Sema-1aP1 | 21 | 45%b | 0% | 32%d |
b dendritic innervation mostly to the region dorsal to the VC1 glomerulus d dendritic arbors around and/or within the VA6 glomerulus |
||
Sema-1aP1+Sema-1a | 5 | 0% | 100%c | 0% | c dendritic innervation largely outside of the DC2 glomerulus (Brp negative region) | ||
VA5 lPN (larval; Fig 4 & S7) |
wt | 7 | 0% | 0% | 0% | ||
Sema-1aP1 | 13 | 31%b | 0% | 23%d |
b dendritic innervation to the region ventral to the VC1 glomerulus d dendritic innervation in part of the VA6 and/or DC3 glomeruli |
||
Sema-1aP1+Sema-1a | 8 | 0% | 100%c | 0% | c dendritic innervation largely outside of the VA5 glomerulus (Brp negative region) | ||
VA7m lPNe (larval; Fig 4 & S7) |
wt | 4 | 0% | 0% | 25%d | d a small patch of dendritic innervation to the VA4 glomerulus | |
Sema-1aP1 | 9 | 44%b | 0% | 67%d |
b dendritic innervation to the region close to the VC1 glomerulus d dendritic innervation in part of the VA4 and/or VC2 glomeruli |
||
Sema-1aP1+Sema-1a | 15 | 0% | 100%c | 0% | c dendritic innervation largely outside of the VA7m glomerulus (Brp negative region) | ||
DA3/VC1 adPNe (emb; Fig 4) |
Sema-1aP1 | 2 | 100%b | 0% | 100%d |
b dendritic innervation to the region around the VC1 glomerulus d heavy dendritic accumulation in the DA3 glomerulus |