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. Author manuscript; available in PMC: 2017 May 11.
Published in final edited form as: Sci Transl Med. 2016 Nov 2;8(363):363ra147. doi: 10.1126/scitranslmed.aac7037

Fig. 4. Single-cell isolation and sequencing of CTCs at low tumor burden.

Fig. 4

(A) Single myeloma CTCs from peripheral blood were isolated from patient P009 at a time point when the patient had a VGPR and no CTCs were detectable by FACS acquiring 100,000 cells (t1, see Table 1). Single CD138+CD45− plasma cells were isolated from the peripheral blood or the BM and the mutational profile was determined as described in Fig. 3. Mutational status in bulk bone marrow by CLIA-certified genotyping is indicated (cc). Bar diagrams (right) represent the fraction of cells harboring the indicated mutations. (B) Mutational profiles of a patient with MGUS in single plasma cells isolated from peripheral blood (red) and BM (blue). Clinical routine test from bulk bone marrow was not performed (*).