Figure 5. CD137 intrinsically promotes the accumulation of IGRP_206-214 reactive CD8 T cells.

Lethally irradiated (NOD x NOD.CD45.2)F1 mice were infused with equal number of T cell depleted BM cells (2.5x10⁶ per donor strain) from NOD.CD45.2 and NOD.Tnfrsf9^−/− (A and C) or NOD.CD45.2 and NOD mice (B and D). The frequencies of CD44^high IGRP_206-214 reactive CD8 T cells were analyzed in the spleens of the F1 recipients by MHC I tetramer staining at 10–12 weeks post BM reconstitution. The origin of the splenocytes was determined by CD45.1 and CD45.2 expression. The gating strategy is shown in panels A and B. The summarized results of two independent experiments are shown in panels C and D. **P < 0.01 by Wilcoxon matched-pairs signed rank test.