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. Author manuscript; available in PMC: 2018 May 15.
Published in final edited form as: J Immunol. 2017 Mar 31;198(10):3857–3868. doi: 10.4049/jimmunol.1601851

Figure 8. Soluble CD137 suppresses CD8 T cell activation.

Figure 8

CD8 T cells purified from 8–10 week-old female NOD mice were labeled by CFSE (final concentration: 0.5 uM), stimulated by 25,000 CD3/CD28 dynabeads and cultured with or without recombinant soluble CD137 (30 ug/ml) on 96-well u-bottom culture plate. After 3 days of culture, CFSE-dilution and 7-AAD positive population was quantified on FACS Calibur. (A) One representative of four experiments for CFSE dilution without soluble CD137 (left) and with soluble CD137 (right) is shown. (B) Soluble CD137 significantly increases the number of undivided CD8 T cells and decreases the number of divided CD8 T cells. Mean percentage of divided and undivided cells is shown (n = 4 experiments). (C) 7-AAD staining shows no difference in CD8 cell death after 3 days of culture with or without soluble CD137 (mean of n = 4 experiments). **P < 0.01; ***P < 0.005 by unpaired t test. sCD137: soluble CD137. NS: not significant.