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. Author manuscript; available in PMC: 2018 May 15.
Published in final edited form as: J Immunol. 2017 Mar 31;198(10):3835–3845. doi: 10.4049/jimmunol.1602122

Figure 5. Fine kinetics of inhibitor blockade.

Figure 5

L-Kb cells were infected with rVVs expressing UbR-NP-S-GFP (A) or NP-S-GFP (B) for 1 h. At 3 h, DMSO, MLN7243, CHX or MG132 were added into cell cultures. Cells were harvested at indicated time points after drug addition. Levels of GFP (left panels) and surface Kb-SIINFEKL (right panels) were determined by flow cytometry. ΔMFI: medium fluorescent intensity after background subtraction.