FIGURE 4:

Loss of cell–cell adhesion causes DC defects and an increase in the amount and stability of cell–ECM adhesions. (a) Sample z-projected images of control and shg^R69b embryos expressing talin-GFP at 1-h intervals during DC. (b) Mean rate of dorsal closure in control and shg^R69b embryos (five embryos). (c–e) FALS area (c), intensity (normalized to cytoplasmic intensity (d), and density (e) in control and shg^R69b embryos (seven or eight embryos, 242–503 FALS). (f) Sample z-projected images of the basal domain of AS cells showing FALS in control and shg^R69b embryos. (g, h) Mean speed (g) and effective diffusion constant (h) of FALS movement in control and shg^R69b embryos (four to seven embryos, 34–58 FALS). Error bars indicate SEM. *p < 0.05, ***p < 0.0001.