FIGURE 5:

Cell oscillatory behavior and force transmission are suppressed in shg^R69b mutants. (a) Temporal overlay of cell contours 20 s apart from time-lapse movies of control and shg^R69b embryos during early DC. (b–d) Mean amplitude (b) and period (c) of apical area oscillations and mean speed of apical cell centroid movement (d) for control and shg^R69b embryos (four embryos, 21–25 cells). (e) Sample images of control and shg^R69b embryos before and after laser ablation. Yellow line indicates cut location, and white arrows indicate tracked first-neighbor junctions. Junction separation at t = 0 (d_{t=0 s}) and 15 s (d_{t=15 s}) are indicated to help visually assess differences in separation over time between samples. (f, g) Recoil velocity (f) and relaxation time (g) of first-neighbor junctions after laser ablation in control and shg^R69b embryos (13–24 cuts). (h) Spatial decay of maximum displacement in control and shg^R69b embryos measured by fitting the maximum displacement of first-neighbor (1^st nbr), second-neighbor (2^nd nbr), and background (Bkgd) junctions to an exponential. R² values and overall spatial decay coefficient (k) are indicated on plots. (i) Mean spatial decay coefficient in controls and shg^R69b embryos. Error bars indicate SEM (unless otherwise indicated). *p < 0.05, ***p < 0.0001.