Figure 5.

Imaging principle of single-molecule localization microscopy. In SMLM, only a small subset of fluorophores can be randomly switched on using appropriate illumination and localized at high resolution; after the small subset of fluorophores is switched off, a new subset is switched on and localized. This cycles is repeated to record many frames, including the localizations of individual fluorophores (a–c). Therefore, an super-resolution image is reconstructed from all of the successful localizations (d). Reproduced and rearranged from [36] with permission.