Fig. 4. miR-383 overexpresssion suppresses in vitro attributes of tumorigenicity in prostate cancer cell lines.
miR-383 mimic/control miR/mock was transfected in Du145/ PC3/ LNCaP cell lines followed by functional assays (performed 72 hrs post-transfection) (*P< .05).
A. Cell cycle assay in Du145/ PC3/ LNCaP cells after miR-CON (left panel) or miR-383 (right panel) transfections.
B. Apoptosis assay in Du145/ PC3/ LNCaP cells after miR-CON (left panel) or miR-383 (right panel) transfections as assessed by ANNEXIN V-FITC /7-AAD staining.
C. MTS cellular viability assays, D. Colony formation assays, and E. Transwell invasion and migration assays in Du145/ PC3/ LNCaP cells transfected with mock/ miR-CON/ miR-383.