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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Matrix Biol. 2016 Nov 11;62:28–39. doi: 10.1016/j.matbio.2016.11.001

Figure 4. HA35 treatment increases ZO-1 expression in both the distal and transverse colon of DSS-treated mice.

Figure 4

A. Groups of mice orally treated with HA35, HA2000 (300µg/mouse) or water once per day for 5 days before, as well as 3 days during 2.5% DSS administration were euthanized and their colons collected for histology. Transverse and distal colon sections were immunostained for ZO-1 (red) and nuclei were stained with DAPI (blue). B. The intensity of ZO-1 staining was quantified using 2 stained sections per mouse, 5 mice per group and normalized to the number of epithelial cells. One-way ANOVA was used to test the statistical significance of differences between HA-treated groups and the water-treated group. (*, p<0.05, **, p<0.01). N.S.=not significant. Error bars=S.E.M.