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Determination of prME protein expression. (A) In vitro expression of the prME protein in DNA-transfected BHK cells by IFA (fluorescence microscopy, × 400). (B) In vivo expression of the prME protein in tissues by IHC. The muscle tissues were collected from mice sacrificed two weeks after the third immunization with 50 μg DNA (light microscopy, × 200).
