Figure 2.

DV1-specific antibody responses in mice sera. (A) Dynamics of the IgG responses detected by ELISA. The mice were immunized three times at two-week intervals. Sera were collected at weeks 0, 2, 4, and 6. All samples were diluted at 1:800 (n = 6). (B) End-point titers of anti-DV1 antibodies assayed by ELISA (n = 6). Sera were collected two weeks after the third immunization. The results were expressed as GMT + standard deviation (SD). (C) The anti-DV1 IgG subclasses in mice sera determined by ELISA. Sera were collected two weeks after the third immunization. Values of IgG2a and IgG1 were reported as the mean OD + SD at 492 nm at a serum dilution of 1:100 (n = 8). (D) Serum NAb responses assayed by PRNT₅₀ (n = 8). Sera were collected two weeks after the third immunization. NAb titers were recorded as GMT + SD. ^*p < 0.05, ^**p < 0.01.