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. 2017 May 12;7:175. doi: 10.3389/fcimb.2017.00175

Figure 3.

Figure 3

The levels of splenocyte-secreted IFN-γ (A), IL-2 (B), IL-4 (C), and IL-10 (D) cytokines by ELISPOT assays (n = 6). Splenocytes were isolated two weeks after the third immunization. The numbers of cytokine-positive cells were recorded as the mean SFU/1 × 106 splenocytes + SD. *p < 0.05; **p < 0.01.