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End-point titers of anti-DV1 antibodies and protect immunity elicited by the pVAX1-D1ME under different times of vaccination (n = 6). (A) End-point titers of anti-DV1 antibodies. (B) Pathological symptoms recorded as the mean sign scores. (C) Body weight calculated as percentages compared with day 0. *p < 0.05, **p < 0.01.
