Figure 2.

Validation data on Forced Swim Test (FST) Scoring. Two experienced raters, after having trained with the Kinoscope program, scored blindly, and independently male and female rats in the 5 min second session of the two-sessions FST. Each animal is represented in a separate row and on each column, the scoring pattern from each blind and independent rater can be seen. Correlation indices were 0.85 for number of recorded behavioral events, 0.98 for immobility behavior (blue color) and 0.90 for immobility latency, 0.89 from swimming (red color), 0.97 for climbing behavior (black color), 0.95 for head shaking frequency (green color). All correlations were highly significant (p < 0.001) as indicated by Pearson's two-tailed test. Full data published in Kokras et al (Kokras et al., 2015). Raw images from Kinoscope were put in order and collated together using ImageJ/Fiji (Schindelin et al., 2012; Schneider et al., 2012).