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A robust dialysis activated Cl– current is recorded from mouse neutrophils lacking a range of Cl– transport pathways but it is much reduced in LRRC8A deficient cells. CLIC1–/–, KCC3–/–, CLCN3–/– and ebo/ebo recordings are from bone marrow derived neutrophils, CLCN7–/– recordings are from mouse blood neutrophils, BEST1 H178P recordings are from human neutrophils. Representative examples, n ≥ 3 for each subtype. Experiments were carried out using solution Set E (Table 1).
