Table 1.
SetA: Unselective solutions, hyperosmotic internal Figure 1) ECl: -11 mv, ΔOsm: 50 mOsm . |
Set B: Unselective solutions, standard internal (Figures 2, 3) ECl: -84 mv, ΔOsm: -10 mOsm . |
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INTERNAL | EXTERNAL | INTERNAL | EXTERNAL |
90 mM KCl | 135 mM NaCl | 27 mM K Aspartate | 140 mM NaCl |
36 mM K Gluconate | 4.7 mM NaOH | 100 mM K Gluconate | 5 mM KCl |
13 mM KOH | 5 mM KCl | 13 mM KOH | 1 mM MgCl 2 |
5 mM NaCl | 0.5 mM MgCl2 | 5 mM NaCl | 1.5 mM MgCl2 |
0.9 mM CaCl2 | 1.8 mM CaCl2 | 0.2 mM CaCl2 | 10 mM Hepes |
2 mM EGTA | 10 mM Hepes | 1 mM EGTA | 5 mM Glucose |
10 mM Hepes | 5 mM Glucose | 20 mM Hepes | |
3 mM Mg ATP | 2 mM Mg ATP | ||
5 mM NADPH | |||
pH 7.35 | pH 7.35 | pH 7.35 | pH 7.35 |
Osm 350 mOsm | Osm 300 mOsm | Osm 290 mOsm | Osm 300 mOsm |
Ionic strength 144 mM | Ionic strength 149 mM | Ionic strength 144 mM | Ionic strength 155 mM |
Set C: Cesium based solutions ECl: -26 mv, ΔOsm: -20 mOsm . |
Set D: NMDG based solutions (Figure 4) ECl: -32 mv, ΔOsm: -10 mOsm . |
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INTERNAL | EXTERNAL | INTERNAL | EXTERNAL |
Low strength | Standard strength | ||
44 mM CsCl | 2 mM NaCl | 155 mM NMDG | 140 mM NMDG |
30 mM CsOH | 132 mM CsCl | 32 mM HCl | 133 mM HCl |
2 mM NaATP | 6 mM CsOH | 105 mM Gluconic acid | 2 mM CaCl2 |
2 mM MgCl2 | 1 mM MgCl2 | 4 mM EGTA | 1 mM MgCl2 |
1 mM CaCl2 | 2 mM CaCl2 | 1 mM CaCl2 | 10 mM Hepes |
10 mM Hepes | 10 mM Hepes | 10 mM Hepes | 5 mM Glucose |
4 mM EGTA | 5 mM Glucose | 2 mM MgCl2 | |
157 mM Sucrose | 2 mM MgATP | ||
pH 7.35 | pH 7.35 | pH 7.35 | pH 7.35 |
Osm 280 mOsm | Osm 300 mOsm | Osm 290 mOsm | Osm 300 mOsm |
Ionic strength 84 mM | Ionic strength 143 mM | Ionic strength 154 mM | Ionic strength 148 mM |
Set E: NMDG based solutions (Figures 4, 5) ECl: -32 mv, ΔOsm: -20 mOsm. | |||
INTERNAL EXTERNAL | |||
Low strength | |||
50 mM NMDG | 140 mM NMDG | ||
32 mM HCl | 133 mM HCl | ||
4 mM EGTA | 2 mM CaCl2 | ||
1 mM CaCl2 | 1 mM MgCl2 | ||
2 mM MgCl2 | 10 mM Hepes | ||
10 mM Hepes | 5 mM Glucose | ||
2 mM MgATP | |||
180 mM Sucrose | |||
pH 7.35 | pH 7.35 | ||
Osm 280 mOsm | Osm 300 mOsm | ||
Ionic strength 53 mM | Ionic strength 148 mM |