Table 1.

Intracellular and extracellular solutions for patch-clamp recordings

SetA: Unselective solutions, hyperosmotic internal Figure 1) ECl: -11 mv, ΔOsm: 50 mOsm .		Set B: Unselective solutions, standard internal (Figures 2, 3) ECl: -84 mv, ΔOsm: -10 mOsm .
INTERNAL	EXTERNAL	INTERNAL	EXTERNAL
90 mM KCl	135 mM NaCl	27 mM K Aspartate	140 mM NaCl
36 mM K Gluconate	4.7 mM NaOH	100 mM K Gluconate	5 mM KCl
13 mM KOH	5 mM KCl	13 mM KOH	1 mM MgCl 2
5 mM NaCl	0.5 mM MgCl2	5 mM NaCl	1.5 mM MgCl2
0.9 mM CaCl2	1.8 mM CaCl2	0.2 mM CaCl2	10 mM Hepes
2 mM EGTA	10 mM Hepes	1 mM EGTA	5 mM Glucose
10 mM Hepes	5 mM Glucose	20 mM Hepes
3 mM Mg ATP		2 mM Mg ATP
5 mM NADPH
pH 7.35	pH 7.35	pH 7.35	pH 7.35
Osm 350 mOsm	Osm 300 mOsm	Osm 290 mOsm	Osm 300 mOsm
Ionic strength 144 mM	Ionic strength 149 mM	Ionic strength 144 mM	Ionic strength 155 mM
Set C: Cesium based solutions ECl: -26 mv, ΔOsm: -20 mOsm .		Set D: NMDG based solutions (Figure 4) ECl: -32 mv, ΔOsm: -10 mOsm .
INTERNAL	EXTERNAL	INTERNAL	EXTERNAL
Low strength		Standard strength
44 mM CsCl	2 mM NaCl	155 mM NMDG	140 mM NMDG
30 mM CsOH	132 mM CsCl	32 mM HCl	133 mM HCl
2 mM NaATP	6 mM CsOH	105 mM Gluconic acid	2 mM CaCl2
2 mM MgCl2	1 mM MgCl2	4 mM EGTA	1 mM MgCl2
1 mM CaCl2	2 mM CaCl2	1 mM CaCl2	10 mM Hepes
10 mM Hepes	10 mM Hepes	10 mM Hepes	5 mM Glucose
4 mM EGTA	5 mM Glucose	2 mM MgCl2
157 mM Sucrose		2 mM MgATP
pH 7.35	pH 7.35	pH 7.35	pH 7.35
Osm 280 mOsm	Osm 300 mOsm	Osm 290 mOsm	Osm 300 mOsm
Ionic strength 84 mM	Ionic strength 143 mM	Ionic strength 154 mM	Ionic strength 148 mM
		Set E: NMDG based solutions (Figures 4, 5) ECl: -32 mv, ΔOsm: -20 mOsm.
		INTERNAL EXTERNAL
		Low strength
		50 mM NMDG	140 mM NMDG
		32 mM HCl	133 mM HCl
		4 mM EGTA	2 mM CaCl2
		1 mM CaCl2	1 mM MgCl2
		2 mM MgCl2	10 mM Hepes
		10 mM Hepes	5 mM Glucose
		2 mM MgATP
		180 mM Sucrose
		pH 7.35	pH 7.35
		Osm 280 mOsm	Osm 300 mOsm
		Ionic strength 53 mM	Ionic strength 148 mM