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. 2017 May 12;8:839. doi: 10.3389/fmicb.2017.00839

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Copyright © 2017 Viducic, Murakami, Amoh, Ono and Miyake.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-dependent killing assay of stationary-phase (A) and logarithmic phase (B) wild-type PAO1 and the ΔrpoN, ΔrpoS, ΔrpoNΔrpoS, and ΔrpoN/rpoN⁺ mutants treated with 32 μg/ml tobramycin grown in LB. Wild-type PAO1 was grown without tobramycin and served as a growth control. Percent survival at the indicated time points was calculated by dividing the number of CFU/ml after antibiotic treatment by the CFU/ml before addition of tobramycin. The experiment was performed in triplicate. Error bars indicate SDs. P ≤ 0.05 (^*), ≤0.01 (^**), or ≤0.001(^***) vs. wild type.