Figure 5.

Recombinant SpTransformer protein, rSpTransformer-E1 (rSpTrf-E1), induces leakage that plateaus at about 5 h. Three independent leakage assays with 10 µM rSpTrf-E1 show that reaching the fluorescence leakage plateau requires about 5 h. The table insert shows that the results are reproducible at 0 initial leakage rate (A₀) when rSpTrf-E1 is added to the sample with average fraction of ~0.58 (A₁) that is released with an average kinetic rate (k) of ~1.17 × 10⁻⁴ s⁻¹.