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. 2017 May 12;8:481. doi: 10.3389/fimmu.2017.00481

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Copyright © 2017 Lun, Samuel, Gillmor, Boyd and Smith.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NBD-PA becomes separated from giant unilamellar vesicles (GUVs) after 2 h of incubation with recombinant SpTransformer protein, rSpTransformer-E1 (rSpTrf-E1). (A,B) NBD-PA (arrows) forms clusters that are separated from the GUVs after 2 h of incubation with rSpTrf-E1. (C,D) GUVs in the absence of rSpTrf-E1 show an even distribution of NBD-PA and DiD at 2 h. Differences in the GUV sizes and content of NBD-PA are an outcome of GUV preparation. All images are merged for NBD-PA (blue) and DiD (red) as captured by confocal microscopy. All scale bars indicate 10 μm.